Pages 376 - 386 Volume 89, Issue 4
Back
0
Download
A
Sensitivity of Anti-PGL-1 Elisa test using mixed antigens (disaccharide + trisaccharide) for the diagnosis and epidemiological surveillance of leprosy
Maria Do Perpétuo Socorro Amador Silvestre
  Maria Do Perpétuo Socorro Amador Silvestre
Affiliation
Instituto Evandro Chagas Ananindeua, Pará, Brazil
 Find this author on Google Scholar
 Find this author on PubMed
a
Maxwell Furtado De Lima
  Maxwell Furtado De Lima
Affiliation
Instituto Evandro Chagas Ananindeua, Pará, Brazil
 Find this author on Google Scholar
 Find this author on PubMed
a
Luana Nepomuceno Gondim Costa Lima
  Luana Nepomuceno Gondim Costa Lima
Affiliation
Instituto Evandro Chagas Ananindeua, Pará, Brazil
Correspondence to: Luana Nepomuceno Lima, Instituto Evandro Chagas Ananindeua, Pará, Brazil (e-mail: luanalima@iec.pa.gov.br)
 Find this author on Google Scholar
 Find this author on PubMed
a,*
Introduction:

The development of immunological and molecular diagnostic tests for leprosy has advanced substantially in the past decade, although such advances have not yet been effective in reducing the burden of leprosy in endemic countries.

 Objective:

To assess the performance of anti-PGL-1 IgM ELISA Mycobacterium leprae test using trisaccharide antigen compared to using mixed antigens (MIX).

 Results:

The average levels of anti-PGL-1 antibodies in multibacillary (MB) patients for NT-P-BSA antigen was 0.494 and the MIX antigens, 0.985; in paucibacillary (PB) patients for NT-P-BSA, 0.06 and the MIX, 0.113; in contacts (CON) the average for NT-P-BSA was 0.088 and the MIX 0.155. Among PB patients, 14% (7/50) were negative for NT-P-BSA and positive for the MIX antigens; among CON 18% (14/78) were negative for NT-P-BSA and positive for the MIX antigens. Analysis of variance showed that the levels of anti-PGL-1 antibodies are significantly higher for the MIX antigens compared to NT-P-BSA.

 Conclusions:

These findings suggest that a test using the mixed antigens to the three classes of immunoglobulins (IgM, IgA and IgG), could represent a more sensitive test for the diagnosis and monitoring of leprosy in endemic countries.
Cite this article
Maria Do Perpétuo Socorro Amador Silvestre, Maxwell Furtado De Lima, Luana Nepomuceno Gondim Costa Lima;
Sensitivity of Anti-PGL-1 Elisa test using mixed antigens (disaccharide + trisaccharide) for the diagnosis and epidemiological surveillance of leprosy; Leprosy Review; 2018; 89; 4; 376-386; DOI: 10.47276/lr.89.4.376
home.article_information

DOI: 10.47276/lr.89.4.376

articleview.journal_title: Leprosy Review
articleview.publisher: British Leprosy Relief Association
articleview.publisher_location: Colchester, UK
articleview.article_date_pre accepted on 22 October 2018 and articleview.pub_on 01 December 2018
© Lepra 2018
LEPROSY
Leprosy Review
0305-7518
British Leprosy Relief Association
Colchester, UK
37-6386
0305-7518/18/064053+11
10.47276/lr.89.4.376
Original Papers
Sensitivity of Anti-PGL-1 Elisa test using mixed antigens (disaccharide + trisaccharide) for the diagnosis and epidemiological surveillance of leprosy
SilvestreMaria Do Perpétuo Socorro Amador
a
LimaMaxwell Furtado De
a
LimaLuana Nepomuceno Gondim Costa
a
a
Instituto Evandro Chagas Ananindeua, Pará, Brazil
Correspondence to: Luana Nepomuceno Lima, Instituto Evandro Chagas Ananindeua, Pará, Brazil (e-mail: )
01122018
89
4
376
386
22102018
© Lepra
2018
Introduction:
The development of immunological and molecular diagnostic tests for leprosy has advanced substantially in the past decade, although such advances have not yet been effective in reducing the burden of leprosy in endemic countries.
Objective:
To assess the performance of anti-PGL-1 IgM ELISA Mycobacterium leprae test using trisaccharide antigen compared to using mixed antigens (MIX).
Results:
The average levels of anti-PGL-1 antibodies in multibacillary (MB) patients for NT-P-BSA antigen was 0.494 and the MIX antigens, 0.985; in paucibacillary (PB) patients for NT-P-BSA, 0.06 and the MIX, 0.113; in contacts (CON) the average for NT-P-BSA was 0.088 and the MIX 0.155. Among PB patients, 14% (7/50) were negative for NT-P-BSA and positive for the MIX antigens; among CON 18% (14/78) were negative for NT-P-BSA and positive for the MIX antigens. Analysis of variance showed that the levels of anti-PGL-1 antibodies are significantly higher for the MIX antigens compared to NT-P-BSA.
Conclusions:
These findings suggest that a test using the mixed antigens to the three classes of immunoglobulins (IgM, IgA and IgG), could represent a more sensitive test for the diagnosis and monitoring of leprosy in endemic countries.
LeprosyAntigenAntibodiesEpidemiology